PCR Advantages: (vs Southern Blot) - simple and faster - requires much less amounts of pathological material - greater quantitative sensitivity - can be applied on DNA paraffin-embedded tissue Disadvantages: (vs Southern Blot) - lower qualitative sensitivity Monoclonality theory. Alternatively, RGB-marked tumor cells were implanted orthotopically. In summary, unequivocal and unjustifiable adherence to the conceptual model that tumors are monoclonal in origin has outlived its usefulness. https://pediaa.com/difference-between-monoclonal-and-polyclonal-antibodies Thus, demonstration of monoclonality is indicative of the presence of a monoclonal B-cell population and polyclonality is indicative of a polyclonal population. Tumors are monoclonal in nature Monoclonality versus Polyclonality Cancer occurs with different frequencies in different populations ? The proportion of histologic B‐cell aggregates was higher in the monoclonality group than in the polyclonality group (37% vs. 3.4%; P < 0.001). Polyclonal theory The occurrence of multiple primary tumors (MPTs) could be attributed to independent molecular events affecting multiple cells in the entire field subsequent to exposure to carcinogens. A Polyclonal Antibody represents a collection of antibodies from different B cells that recognize multiple epitopes on the same antigen. Chimeric models have yielded direct unequivocal demonstrations of polyclonality in rodent and human tumors. Overall, 9.4% (n = 37) showed both monoclonality and histologic B-cell aggregates, 16% (n = 63) monoclonality only, 2.5% (n = 10) histologic B-cell aggregates only, and 72.1% ( n = 284) no abnormality. Background/Aims— Detection of clonal immunoglobulin heavy chain (IgH) rearrangements by the polymerase chain reaction (PCR) is an attractive alternative to Southern blotting in lymphoma diagnostics. polyclonality monoclonality. However, the advantages and limitations of PCR in clonality analysis are still not fully appreciated. d Quantification of metastatic nodules (n = 146) according to monoclonality and polyclonality (left graph, P value = 0.0497) and further categorized … Also, mutational data are consistent with polyclonal tumor origin. that the study aimed to evaluate the dichotomy of monoclonality vs. polyclonality; sample size reduction/increase was dependent on cell line tumorigenicity. When a breast cancer cell metastasizes to lung. Monoclonality definition, the state or condition of having one specific type of antibody. If polyclonality is, indeed, more common than monoclonality, then combination therapies may be more appropriate, in general, than monotherapies. 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